Chromogenic substrate for detection of a-galactosidase activity in yeast Saccharomyces cerevisiae. Used in yeast Two-Hybrid System to detect protein-protein interactions within random libraries. The enzyme a-galactosidase enabels yeast to use disaccharide melibose as a carbon source during growth or fermentation. Secretion of this enzyme in response to activation of the GAL4 gene hydrolizes X-a-GAL and causes yeast colonies to develop a blue color. This reporter system allows immediate visible results on selection plates and eliminates necessity of a second screen filter life assay.
Substance Name: 5-Bromo-4-Chloro-3-indolyl-a-D-galactopyranoside
CAS Number: 107021-38-5
Molecular Formula: C14H15BrClNO6
Molecular Weight: 408.6
Chemical Purity: >98%
Soluble in: DMSO and DMF
Store at: 2-8°C
Common Name: 5-Bromo-4-Chloro-3-indolyl-a-D-galactopyranoside
Substance Name: 5-Bromo-4-Chloro-3-indolyl-a-D-galactopyranoside
CAS Number: 107021-38-5
Molecular Formula: C14H15BrClNO6
Molecular Weight: 408.6
Chemical Purity: >98%
Soluble in: DMSO and DMF
Store at: 2-8°C
Common Name: 5-Bromo-4-Chloro-3-indolyl-a-D-galactopyranoside
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