X-a-Gal, 1 G

Chromogenic substrate for detection of a-galactosidase activity in yeast Saccharomyces cerevisiae. Used in yeast Two-Hybrid System to detect protein-protein interactions within random libraries. The enzyme a-galactosidase enabels yeast to use disaccharide melibose as a carbon source during growth or fermentation. Secretion of this enzyme in response to activation of the GAL4 gene hydrolizes X-a-GAL and causes yeast colonies to develop a blue color. This reporter system allows immediate visible results on selection plates and eliminates necessity of a second screen filter life assay. Common Name: 5-Bromo-4-Chloro-3-indolyl-a-D-galactopyranoside CAS Number: 107021-38-5 Molecular Weight: 408.6 Chemical Formula: C14H15BrClNO6 Solubility: DMSO and DMF Storage Temperature: -20C Storage Conditions: Freezer Appearance: Colorless or white to off- white crystalline powder Solution Test (1% in Methanol): Clear and colorless Turbidity (1% in Methanol): 4.0 NTU Water (KF): 1.0 % Purity (TLC)