X-GlcA Cyclohexylammonium Salt, 1 G

Histochemical substrate for β-D-Glucuronidase (GUS) encoded by the gusA gene. The substrate is used as a qualitative histochemical marker of specific GUS expression in cells and tissue. X-GlcA is cleaved by GUS at the B1 glucuronic bond between glucuronic acid and the 5-Bromo-4-chloro-3-indolyl part of X-GlcA via hydrolysis. The cleavage of X-GlcA results in the precipitation of water insoluble blue dichloro-dibromo-indigo precipitate at the site of enzymatic cleavage.Allow to warm to room temperature before using. Common Name: 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid, cyclohexylammonium salt CAS Number: 114162-64-0 Molecular Weight: 521.8 Chemical Formula: C20H26BrClN2O7 Solubility: DMF and DMSO Storage Temperature: 2-8C Storage Conditions: Refrigerator Appearance: White to off white powder Identity: 1H NMR Conforms to structure Purity: 98 % Solution Test (2% in DMF): Clear, Colorless Specific Rotation: -92 - -88 Water Content (K.F): 3.0 % Shipping Weight: 0.0399 lbs S