Cystine Heart Agar, 500 G

Supports excellent growth of gram-negative cocci and other pathogenic organisms. With added hemoglobin it is used for cultivation of Francisella tularensis.Prepare medium by mixing 51 grams of agar medium in 1 Liter of purified water. Mix, heat and autoclave. Cool to 50-60°C. Add 100ml sterile 2% hemoglobin solution. Dispense into sterile petri dish or tube.Formula per Liter:Beef Heart Infusion: 10 gProteose Peptone: 10 gDextrose: 10 gSodium Chloride: 5 gL-Cystine: 1 gAgar: 15 g Solubility: Water with Heat Storage Temperature: Room Temperature Storage Conditions: Room Temperature Appearance: Homogeneous Free Flowing,Beige Powder pH (5.1 % Solution @25 C): 6.6 - 7.0 Microorganism (Neisseria meningitides ATCC 13090): Fair to Excellent Microorganism (Staphylococcus Aureus ATCC 25923): Fair to Excellent Microorganism (Streptococcus pneumoniae ATCC 6305): Fair to Excellent Agar: 15.0 g/l Beef Heart Infusion: 10.0 g/l Dextrose: 10.0 g/l Proteose Peptone: 10.0 g/l Sodium Chloride: 5.0